in situ hybridization
对于极微量的靶序列的扩增可以采用的PCR技术是A、通用引物-PCR方法(general primer-media-ted PCR，GP-PCR)B、多重PCR(multiplex PCR)C、套式PCR(nested primers-polymerase chain reaction，NP-PCR)D、AP-PCR方法(arbitrarily primed PCR)E、原位PCR技术(in situ PCR，ISPCR)
点击查看答案
对于极微量的靶序列的扩增可以采用下列哪种PCR技术？( )A、AP-PCR方法(arbitrarily primed PCR)B、通用引物-PCR方法(general primer-mediated PCR，GP-PCR)C、套式PCR(nested primers-polymerase chainreaction，NP-PCR)D、多重PCR(multiplex PCR)E、原位PCR技术(in situ PCR，ISPCR)
The role of women in Britain has changed a lot in this century, () in the last twenty years. The main change has been () giving women greater equality with men. Up to the beginning of this century, women seem to have had () rights. They could not vote and were kept at home. () , as far as we know, most women were happy with this situ ation. Today, women in Britain certainly () more rights than they used to. They were () the vote in 1919. In 1970 a law was passed to give them an equal () of wealth in the case of divorce, () the Equal Pay Act gave them the right of equal pay with men for work of equal value in the same year. Yet () these changes, there are still great difference in status between men and women. Many employers seem to () the Equal Pay Act, and the average working women is () to earn only about half that a man earns for the same job. () a survey, at present, only one-third of the country’s workers are () women. This small percentage is partly () a shortage of nurseries. If there were () nurseries, twice as many women might well go out to workA.butB.andC.becauseD.although
第二组The only attention that heat exchanger should require is to ensure that the heat transfer surfaces remain substantially clean and the flow passages generally clear of obstruction. Indication that undue fouling is occurring is given by a progressive increase in the temperature difference between the two fluids, over a period of time, usually accompanied by a noticeable rise in pressure loss at a given flow.Fouling on the sea-water side is the most usual cause of deterioration in performance. The method of cleaning the sea water-side surfaces depends on the type of heat exchanger. With shell-and-tube heat exchangers, the removal of the header covers or, m the case of the smaller heat exchangers, the headers themselves, will provide access to the tubes. Obstructions, dirt, scale etc., can then be removed, using the tools provided by the heat exchanger manufacturer. Flushing through with fresh water is recommended before a heat exchanger is returned to service. In some applications, such as piston oil cooling, progressive fouling may take place on the outside of the tubes. Most manufactures recommend a chemical flushing pr6cess to remove this in situ~ without dismantling the heat exchanger.Plate heat exchangers may be cleaned by unclamping the stack of plates and mechanically cleaning the surface of each plate as recommended by the manufacturers. The plate seals may require replacement from time to time and here the manufacturers&39; instructions should be closely followedBy which of the following indication, can we conclude that the lube oil-sea water heat exchanger is unduly fouled?A.the heat exchanger lube oil inlet temperature loweringB.the heat exchanger sea-water outlet pressure riseC.the heat exchanger temperature difference between lube oil and sea water increasingD.the heat exchanger pressure difference between sea water inlet and outlet decreasing
与蛋白质芯片技术原理相似的技术是（）。A、Northern blottingB、Western blottingC、Southern blottingD、Eastern blottingE、dot blot hybridization
RNA驱动杂交（driven hybridization）
原位杂交（in situ hybridization）技术常用于（）A、转基因B、基因定位C、基因扩增
